WHSC1 Protein (DYKDDDDK Tag)
Quick Overview for WHSC1 Protein (DYKDDDDK Tag) (ABIN2669701)
Target
See all WHSC1 ProteinsProtein Type
Origin
Source
Application
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Purification tag / Conjugate
- This WHSC1 protein is labelled with DYKDDDDK Tag.
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Characteristics
- Recombinant MMSET / WHSC1 (accession number NP_579877.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 154 kDa.
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Wolf-Hirschhorn Syndrome Candidate 1 (WHSC1) (AA 1-1365) protein (His tag)
custom-made WHSC1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Wolf-Hirschhorn Syndrome Candidate 1 (WHSC1) (AA 1-1365) protein (His tag)custom-made WHSC1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Wolf-Hirschhorn Syndrome Candidate 1 (WHSC1) (AA 1-647) protein (GST tag)WHSC1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Wolf-Hirschhorn Syndrome Candidate 1 (WHSC1) (AA 941-1240) protein (His tag)WHSC1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Wolf-Hirschhorn Syndrome Candidate 1 (WHSC1) (AA 942-1240) protein (GST tag)WHSC1 Origin: Human Host: Escherichia coli (E. coli) Recombinant EAA, ScA
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Application Notes
- Recombinant MMSET / WHSC1 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K36me2 methyltransferase activity. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Nucleosomes, 30 ng/μl Recombinant MMSET / WHSC1 protein at 2 hours at room temperature. Activity was detected by fluorography.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1 (WHSC1))
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Alternative Name
- MMSET / WHSC1
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Background
- MMSET (Multiple Myeloma SET domain-containing protein) / WHSC1 (Wolf-Hirschhorn Syndrome Candidate 1, also known as NSD2, Nuclear Receptor binding SET domain protein 2, TRX5, Trithorax 5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The MMSET / WHSC1 gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15 % of patients with Multiple Myeloma (MM), a chromosomal translocation leads to MMSET / WHSC1 gene being aberrantly placed under the control of the IgH intronic μ enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in MMSET / WHSC1 expression in a myeloma cell line suppressed its growth, suggesting a role for MMSET in maintaining the transformed state.
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Molecular Weight
- 154 kDa
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Pathways
- SARS-CoV-2 Protein Interactome
Target
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