CD1d:Ig Fusion Protein Protein

Catalog No. ABIN2688825

This Recombinant CD1d:Ig Fusion Protein protein is produced in Escherichia coli (E. coli). There are 6 publications available.

Quick Overview for CD1d:Ig Fusion Protein Protein (ABIN2688825)

Target: CD1d:Ig Fusion Protein

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS), ELISA

Product Details

Characteristics: The CD1d:Ig fusion protein consists of the extracellular major histocompatibility complex (MHC) class I-like domains of the human CD1d molecule fused with the VH regions of mouse IgG1. Like MHC class I molecules, the CD1d noncovalently associates with β2 Microglobulin (β2M). For this reason, BD Pharmingen™ DimerX consists of recombinant CD1d:Ig and human β2M. Recombinant CD1d molecules, like the DimerX fusion protein, are useful for studying Natural Killer T (NKT)-cell function by immunofluorescent staining and flow cytometric analysis of antigen-specific NKT cells. The human CD1D1 gene encodes a non-polymorphic cell-surface protein that plays a role in antigen presentation to CD1d-restricted NKT cells. Like the MHC class I molecules, CD1d associates noncovalently with β2M and is capable of binding and presenting lipid antigens. While the natural ligand for CD1d is presently unknown, it is well documented that CD1d can bind and present the glycolipid, α-galactosyl ceramide (α-GalCer*), a glycosphingolipid from the marine sponge. Glycosylphosphatidylinositol (GPI) has also been identified as a major CD1d-associated component. Antigenic glycolipids such as α-GalCer associated with the CD1d molecule are presented and specifically recognized by NKT cells expressing a highly conserved TCR, Vα24JαQ, paired with Vβ 11. The human CD1d-α-GalCer complex has been shown to stain a subpopulation of Vα 24+ cells. The human CD1d:Ig dimer presenting the α-GalCer molecule has been shown to stain a higher percentage of Vα 14Ja281+Vβ8.2+ NKT cells in the mouse than Vα 14+Vβ 7+ NKT cells. However, the percentage of mouse NKT cells stained by the human CD1d:Ig-α-GalCer complex was significantly lower than the percentage stained by mouse CD1d:Ig-α-GalCer. The function and lineage marker expression of NKT cells and their interaction with CD1d has been recently reviewed. Schematic representation of the CD1d:Ig dimeric protein

Purification: The human CD1d:Ig fusion protein was expressed together with human β2M in the mouse plasmacytoma cell line, J558L (ATCC TIB-6). The CD1d and β2M polypeptide chains are associated noncovalently as a consequence of their coexpression within J558L cells. The CD1d:Ig fusion protein was purified from tissue culture supernatant by affinity chromatography.The purity of the preparation was confirmed by SDS-PAGE. Additionally, human β2M was added to the reagent. The human CD1d:Ig is tested by ELISA to verify the fusion protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD™ DimerX: Recombinant Soluble Dimeric Human CD1d:Ig Fusion Protein - Purified

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Schümann, Voyle, Wei, MacDonald: "Cutting edge: influence of the TCR V beta domain on the avidity of CD1d:alpha-galactosylceramide binding by invariant V alpha 14 NKT cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 5815-9, (2003) (PubMed).

Lee, Benlagha, Teyton, Bendelac: "Distinct functional lineages of human V(alpha)24 natural killer T cells." in: The Journal of experimental medicine, Vol. 195, Issue 5, pp. 637-41, (2002) (PubMed).

Sidobre, Kronenberg: "CD1 tetramers: a powerful tool for the analysis of glycolipid-reactive T cells." in: Journal of immunological methods, Vol. 268, Issue 1, pp. 107-21, (2002) (PubMed).

Godfrey, Hammond, Poulton, Smyth, Baxter: "NKT cells: facts, functions and fallacies." in: Immunology today, Vol. 21, Issue 11, pp. 573-83, (2001) (PubMed).

Karadimitris, Gadola, Altamirano, Brown, Woolfson, Klenerman, Chen, Koezuka, Roberts, Price, Dusheiko, Milstein, Fersht, Luzzatto, Cerundolo: "Human CD1d-glycolipid tetramers generated by in vitro oxidative refolding chromatography." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 6, pp. 3294-8, (2001) (PubMed).

Naidenko, Maher, Ernst, Sakai, Modlin, Kronenberg: "Binding and antigen presentation of ceramide-containing glycolipids by soluble mouse and human CD1d molecules." in: The Journal of experimental medicine, Vol. 190, Issue 8, pp. 1069-80, (1999) (PubMed).

Target Details

Target: CD1d:Ig Fusion Protein

Sub Type: Fusionprotein