H-2K[b]:Ig Fusion Protein Protein

Catalog No. ABIN2688827

Recombinant H-2K[b]:Ig Fusion Protein protein expressed in Escherichia coli (E. coli) and has been mentioned in 4+ publications.

Quick Overview for H-2K[b]:Ig Fusion Protein Protein (ABIN2688827)

Target: H-2K[b]:Ig Fusion Protein

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Product Details

Characteristics: The H-2D[b]:Ig fusion protein consists of three extracellular major histocompatibility complex (MHC) class I H-2D[b] domains that are fused to the VH regions of mouse IgG1 (see Figure 1). In order for the MHC class I to be functional, ie, capable of binding peptides, ß2 Microglobulin (ß2M) must be present. For this reason, BD™ DimerX consists of recombinant H-2D[b]:Ig fusion protein, supplemented with recombinant ß2M. Recombinant MHC molecules, like the DimerX fusion protein, are useful for studying T-cell function by immunofluorescent staining and flow cytometric analysis of antigen-specific T cells. The MHC gene locus encodes a group of highly polymorphic, cell-surface proteins that play a broad role in the immune response to protein antigens. MHC molecules function by binding and presenting small antigenic protein fragments to antigen-specific receptors expressed by T cells (TCR). Human (human leukocyte antigen/HLA) and mouse (histocompatibility 2/H-2) MHC molecules are structurally and functionally related proteins that comprise two major classes. Class I MHC molecules consist of two separate polypeptide chains. The class I α chain is an MHC encoded, transmembrane polypeptide containing three extracellular domains: α1, α2, and α3. The second chain consists of a non-MHC encoded polypeptide called ß2M. Since ß2M does not contain a transmembrane domain, it associates with the achain through noncovalent interaction. Functionally, class I MHC molecules can bind peptides derived from intracellular antigens (eg, viral and some bacterial antigens) that are specifically recognized by CD8+ T cells. Class II MHC molecules consist of two different transmembrane proteins that can bind peptide fragments derived from extracellular proteins (eg, bacteria and fungi) and are specifically recognized by CD4+ T cells. TCR recognize both processed peptides bound to MHC, as well as regions of the MHC molecule itself. CD4 and CD8 accessory molecules strengthen formation of the TCR-MHC complex through their interaction with non-polymorphic regions of the MHC molecule.

Purification: Purified

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD™ DimerX: Recombinant Soluble Dimeric Mouse H-2D[b]:Ig Fusion Protein - Purified - Clone DimerX/H-2Db - Isotype Mouse IgG1, λ

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Selin, Lin, Kraemer, Pardoll, Schneck, Varga, Santolucito, Pinto, Welsh: "Attrition of T cell memory: selective loss of LCMV epitope-specific memory CD8 T cells following infections with heterologous viruses." in: Immunity, Vol. 11, Issue 6, pp. 733-42, (2000) (PubMed).

Murali-Krishna, Altman, Suresh, Sourdive, Zajac, Miller, Slansky, Ahmed: "Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection." in: Immunity, Vol. 8, Issue 2, pp. 177-87, (1998) (PubMed).

Mendel, Kerlero de Rosbo, Ben-Nun et al.: "A myelin oligodendrocyte glycoprotein peptide induces typical chronic experimental autoimmune encephalomyelitis in H-2b mice: fine specificity and T cell receptor V beta expression of ..." in: European journal of immunology, Vol. 25, Issue 7, pp. 1951-9, (1995) (PubMed).

Rammensee, Friede, Stevanoviíc: "MHC ligands and peptide motifs: first listing." in: Immunogenetics, Vol. 41, Issue 4, pp. 178-228, (1995) (PubMed).

Target Details

Target: H-2K[b]:Ig Fusion Protein

Sub Type: Fusionprotein