H-2Ld:Ig Fusion Protein Protein

Catalog No. ABIN2688829

This Recombinant H-2Ld:Ig Fusion Protein protein is expressed in Escherichia coli (E. coli) and has been mentioned in 2+ publications.

Quick Overview for H-2Ld:Ig Fusion Protein Protein (ABIN2688829)

Target: H-2Ld:Ig Fusion Protein

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Product Details

Characteristics: The H-2Ld:Ig fusion protein consists of three extracellular major histocompatibility complex (MHC) class I H-2Ld domains that are fused to the VH regions of mouse IgG1 (see Figure 1). In order for the MHC class I to be functional, i.e., capable of binding peptides, β2 Microglobulin (β2M) must be present. For this reason, the BD Pharmingen DimerX consists of recombinant H-2Ld:Ig fusion protein, supplemented with recombinant β2M. Recombinant MHC molecules, like the DimerX fusion protein, are useful for studying T-cell function by immunofluorescent staining and flow cytometric analysis of antigen-specific T cells. The MHC gene locus encodes a group of highly polymorphic, cell-surface proteins that play a broad role in the immune response to protein antigens. MHC molecules function by binding and presenting small antigenic protein fragments to antigen-specific receptors expressed by T cells (TCR). Human (human leukocyte antigen/HLA) and mouse (histocompatibility 2/H-2) MHC molecules are structurally and functionally related proteins that comprise two major classes. Class I MHC molecules consist of two separate polypeptide chains. The class I α chain is an MHC encoded, transmembrane polypeptide containing three extracellular domains: α1, α2, and α3. The second chain consists of a non-MHC encoded polypeptide called β2M. Since β2M does not contain a transmembrane domain, it associates with the α chain through noncovalent interaction. Functionally, class I MHC molecules can bind peptides derived from intracellular antigens (e.g., viral and some bacterial antigens) that are specifically recognized by CD8+ T cells. Class II MHC molecules consist of two different transmembrane proteins that can bind peptide fragments derived from extracellular proteins (e.g., bacteria and fungi) and are specifically recognized by CD4+ T cells. TCR recognize both processed peptides bound to MHC, as well as regions of the MHC molecule itself. CD4 and CD8 accessory molecules strengthen formation of the TCR-MHC complex through their interaction with non-polymorphic regions of the MHC molecule.

Purification: The H-2Ld protein was expressed together with human β2M in the mouse plasmacytoma cell line, J558L (ATCC TIB-6). The H-2Ld and β2M polypeptide chains are associated noncovalently as a consequence of their coexpression within J558L cells. The H-2Ld:Ig fusion protein was purified from tissue culture supernatant by affinity chromatography.

Application Details

Comment:

BD™ DimerX:Recombinant Soluble Dimeric Mouse H-2Ld:Ig Fusion Protein - Purified

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Cai, Brunmark, Luxembourg, Garcia, Degano, Teyton, Wilson, Peterson, Sprent, Jackson: "Probing the activation requirements for naive CD8+ T cells with Drosophila cell transfectants as antigen presenting cells." in: Immunological reviews, Vol. 165, pp. 249-65, (1999) (PubMed).

Cai, Kishimoto, Brunmark, Jackson, Peterson, Sprent: "Requirements for peptide-induced T cell receptor downregulation on naive CD8+ T cells." in: The Journal of experimental medicine, Vol. 185, Issue 4, pp. 641-51, (1997) (PubMed).

Target Details

Target: H-2Ld:Ig Fusion Protein

Sub Type: Fusionprotein