Caspase 3 Protein (CASP3)

Catalog No. ABIN2688833

Recombinant Caspase 3 protein expressed in Escherichia coli (E. coli).

Quick Overview for Caspase 3 Protein (CASP3) (ABIN2688833)

Target: Caspase 3 (CASP3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Fluorescence Spectroscopy (FS)

Purity: ≥ 95%

Product Details

Brand: BD Pharmingen™

Purification: Active caspase-3 was expressed in E.coli and purified. When expressed in E. coli, caspase-3 will spontaneously undergo autoprocessing to yield the subunits characteristic of the active enzyme. Cleavage of the Ac-DEVD-AMC substrate by recombinant human caspases-3, -6, -7, and -8. The activity of the caspases was analyzed by spectrofluorometry using an excitation at 380 nm and an emission wwavelength of 430-460 nm (peak is at 440 nm).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

BD Pharmingen™ Recombinant Human Active Caspase-3

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 50 mM Tris ( pH 8.0) with 100 mM NaCl and 50 mM imidazole

Storage: 4 °C,-80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. The thawed active enzyme is generally stable at 4°C for about one week.

Target Details

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation