Caspase 3 Protein (CASP3) (AA 29-277) (His-IF2DI Tag)

Catalog No. ABIN7122165

This Recombinant Caspase 3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Caspase 3 Protein (CASP3) (AA 29-277) (His-IF2DI Tag) (ABIN7122165)

Target: Caspase 3 (CASP3)

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 29-277

Purification tag / Conjugate: This Caspase 3 protein is labelled with His-IF2DI Tag.

Purpose: Recombinant Mouse casp3

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: Caspase 3 (CASP3)

Alternative Name: casp3

Background: Apopain, CASP 3, CASP3, Caspase 3, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

Molecular Weight: 49 kDa

UniProt: P70677

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation