Quick Overview for DAAM1 Protein (Myc-DYKDDDDK Tag) (ABIN2712410)
Target
See all DAAM1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This DAAM1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human DAAM1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) (AA 1-1077) protein (His tag)
custom-made DAAM1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Dishevelled Associated Activator of Morphogenesis 1 (DAAM1) (AA 1-1078) protein (His tag)custom-made DAAM1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- DAAM1 (Dishevelled Associated Activator of Morphogenesis 1 (DAAM1))
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Alternative Name
- Daam1
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Background
- Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins.
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Molecular Weight
- 123.3 kDa
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NCBI Accession
- NP_055807
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Pathways
- WNT Signaling
Target
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