DAAM1 Protein (AA 1-1078) (His tag)

Catalog No. ABIN7553620

This Recombinant DAAM1 protein is produced in HEK-293 Cells.

Quick Overview for DAAM1 Protein (AA 1-1078) (His tag) (ABIN7553620)

Target: DAAM1 (Dishevelled Associated Activator of Morphogenesis 1 (DAAM1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1078

Purification tag / Conjugate: This DAAM1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant DAAM1 Protein expressed in mammalian cells.

Sequence: MAPRKRGGRG ISFIFCCFRN NDHPEITYRL RNDSNFALQT MEPALPMPPV EELDVMFSEL VDELDLTDKH REAMFALPAE KKWQIYCSKK KDQEENKGAT SWPEFYIDQL NSMAARKSLL ALEKEEEEER SKTIESLKTA LRTKPMRFVT RFIDLDGLSC ILNFLKTMDY ETSESRIHTS LIGCIKALMN NSQGRAHVLA HSESINVIAQ SLSTENIKTK VAVLEILGAV CLVPGGHKKV LQAMLHYQKY ASERTRFQTL INDLDKSTGR YRDEVSLKTA IMSFINAVLS QGAGVESLDF RLHLRYEFLM LGIQPVIDKL REHENSTLDR HLDFFEMLRN EDELEFAKRF ELVHIDTKSA TQMFELTRKR LTHSEAYPHF MSILHHCLQM PYKRSGNTVQ YWLLLDRIIQ QIVIQNDKGQ DPDSTPLENF NIKNVVRMLV NENEVKQWKE QAEKMRKEHN ELQQKLEKKE RECDAKTQEK EEMMQTLNKM KEKLEKETTE HKQVKQQVAD LTAQLHELSR RAVCASIPGG PSPGAPGGPF PSSVPGSLLP PPPPPPLPGG MLPPPPPPLP PGGPPPPPGP PPLGAIMPPP GAPMGLALKK KSIPQPTNAL KSFNWSKLPE NKLEGTVWTE IDDTKVFKIL DLEDLERTFS AYQRQQDFFV NSNSKQKEAD AIDDTLSSKL KVKELSVIDG RRAQNCNILL SRLKLSNDEI KRAILTMDEQ EDLPKDMLEQ LLKFVPEKSD IDLLEEHKHE LDRMAKADRF LFEMSRINHY QQRLQSLYFK KKFAERVAEV KPKVEAIRSG SEEVFRSGAL KQLLEVVLAF GNYMNKGQRG NAYGFKISSL NKIADTKSSI DKNITLLHYL ITIVENKYPS VLNLNEELRD IPQAAKVNMT ELDKEISTLR SGLKAVETEL EYQKSQPPQP GDKFVSVVSQ FITVASFSFS DVEDLLAEAK DLFTKAVKHF GEEAGKIQPD EFFGIFDQFL QAVSEAKQEN ENMRKKKEEE ERRARMEAQL KEQRERERKM RKAKENSEES GEFDDLVSAL RSGEVFDKDL SKLKRNRKRI TNQMTDSSRE RPITKLNF Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DAAM1 (Dishevelled Associated Activator of Morphogenesis 1 (DAAM1))

Alternative Name: DAAM1

Background: Disheveled-associated activator of morphogenesis 1,FUNCTION: Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia (PubMed:16630611, PubMed:17482208). Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity). {ECO:0000250|UniProtKB:B0DOB5, ECO:0000250|UniProtKB:Q8BPM0, ECO:0000269|PubMed:16630611, ECO:0000269|PubMed:17482208}.

Molecular Weight: 123.5 kDa

UniProt: Q9Y4D1

AlphaFold: Q9Y4D1

Pathways: WNT Signaling