CYP21A2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for CYP21A2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2712755)
Target
See all CYP21A2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This CYP21A2 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human CYP21A2 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2) (AA 1-487) protein (His tag)
custom-made CYP21A2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2) (AA 1-495) protein (GST tag)CYP21A2 Origin: Human Host: Wheat germ Recombinant AbP, ELISA, PAr, WB
Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2) (AA 1-495), (full length) protein (His tag)CYP21A2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2) (AA 2-261) protein (His tag)CYP21A2 Origin: Rat Host: Yeast Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2) (AA 2-261) protein (His tag)CYP21A2 Origin: Human Host: Yeast Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2) (AA 2-261) protein (His-SUMO Tag)CYP21A2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CYP21A2 (Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2))
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Alternative Name
- Cyp21a2
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Background
- This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 55.8 kDa
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NCBI Accession
- NP_000491
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Pathways
- Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process
Target
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