Quick Overview for CYP2E1 Protein (Myc-DYKDDDDK Tag) (ABIN2712770)
Target
See all CYP2E1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This CYP2E1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human CYP2E1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (AA 1-493) protein (His tag)
custom-made CYP2E1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (AA 1-493) protein (GST tag)CYP2E1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (AA 245-493) protein (His-IF2DI Tag)CYP2E1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))
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Alternative Name
- Cyp2e1
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Background
- This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
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Molecular Weight
- 56.7 kDa
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NCBI Accession
- NP_000764
Target
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