Keratin 6 Protein (KRT6) (His tag)

Catalog No. ABIN2712834

This Recombinant Keratin 6 protein is produced in Escherichia coli (E. coli). There is 1 publication available.

Quick Overview for Keratin 6 Protein (KRT6) (His tag) (ABIN2712834)

Target: Keratin 6 (KRT6)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Keratin 6 protein is labelled with His tag.

Characteristics:

• Recombinant human Cytokeratin 6 (full length, N-term HIS tag) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Yang, Li, Tang, Lin, Wu, Bao: "Smartphone-Based Quantitative Analysis of Protein Array Signals for Biomarker Detection in Lupus." in: Chemosensors (Basel, Switzerland), Vol. 10, Issue 8, (2022) (PubMed).

Target Details

Target: Keratin 6 (KRT6)

Alternative Name: Cytokeratin 6

Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Molecular Weight: 60

NCBI Accession: NP_005545