Factor VII Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2712925

This Recombinant Factor VII protein is expressed in HEK-293 Cells.

Quick Overview for Factor VII Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2712925)

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This Factor VII protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Coagulation factor VII (F7) (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Factor VII (F7) (Coagulation Factor VII (F7))

Background: This gene encodes coagulation factor VII which is a vitamin K-dependent factor essential for hemostasis. This factor circulates in the blood in a zymogen form, and is converted to an active form by either factor IXa, factor Xa, factor XIIa, or thrombin by minor proteolysis. Upon activation of the factor VII, a heavy chain containing a catalytic domain and a light chain containing 2 EGF-like domains are generated, and two chains are held together by a disulfide bond. In the presence of factor III and calcium ions, the activated factor then further activates the coagulation cascade by converting factor IX to factor IXa and/or factor X to factor Xa. Defects in this gene can cause coagulopathy. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

Molecular Weight: 28 kDa

NCBI Accession: NP_062562

Pathways: Response to Growth Hormone Stimulus, Platelet-derived growth Factor Receptor Signaling