COASY Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
Quick Overview for COASY Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag) (ABIN2712941)
Target
See all COASY ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 3
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Purification tag / Conjugate
- This COASY protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Coenzyme A synthase (COASY), nuclear gene encoding mitochondrial protein, transcript variant 3 (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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CoA Synthase (COASY) (AA 1-564) protein (His tag)
custom-made COASY Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CoA Synthase (COASY) (AA 1-563) protein (His tag)custom-made COASY Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
CoA Synthase (COASY) (AA 1-225) protein (GST tag)COASY Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
CoA Synthase (COASY) (AA 296-564) protein (His-IF2DI Tag)COASY Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
CoA Synthase (COASY) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)COASY Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
CoA Synthase (COASY) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)COASY Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- COASY (CoA Synthase (COASY))
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Alternative Name
- Coenzyme A Synthase (Coasy)
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Background
- Coenzyme A (CoA) functions as a carrier of acetyl and acyl groups in cells and thus plays an important role in numerous synthetic and degradative metabolic pathways in all organisms. In eukaryotes, CoA and its derivatives are also involved in membrane trafficking and signal transduction. This gene encodes the bifunctional protein coenzyme A synthase (CoAsy) which carries out the last two steps in the biosynthesis of CoA from pantothenic acid (vitamin B5). The phosphopantetheine adenylyltransferase domain of this bifunctional protein catalyzes the conversion of 4'-phosphopantetheine into dephospho-coenzyme A (dpCoA) while its dephospho-CoA kinase domain completes the final step by phosphorylating dpCoA to form CoA. Mutations in this gene are associated with neurodegeneration with brain iron accumulation (NBIA). Alternative splicing results in multiple isoforms.
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Molecular Weight
- 62.1 kDa
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NCBI Accession
- NP_001035995
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Pathways
- Ribonucleoside Biosynthetic Process
Target
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