CA8 Protein (Myc-DYKDDDDK Tag)
Quick Overview for CA8 Protein (Myc-DYKDDDDK Tag) (ABIN2713137)
Target
See all CA8 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This CA8 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Carbonic anhydrase 8 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Carbonic Anhydrase VIII (CA8) (AA 1-290) protein (GST tag)
CA8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase VIII (CA8) (AA 1-290) protein (His-IF2DI Tag)CA8 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Carbonic Anhydrase VIII (CA8) (Active) protein (His tag)CA8 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 88 % as determined by reducing SDS-PAGE. Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CA8 (Carbonic Anhydrase VIII (CA8))
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Alternative Name
- Carbonic Anhydrase 8
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Background
- The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants.
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Molecular Weight
- 32.8 kDa
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NCBI Accession
- NP_004047
Target
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