ITGAL Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2713386

This Recombinant ITGAL protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for ITGAL Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2713386)

Target: ITGAL (Integrin alpha-L (ITGAL))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This ITGAL protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human CD11a / ITGAL (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

De Franceschi, Peuhu, Parsons, Rissanen, Vattulainen, Salmi, Ivaska, Pouwels: "Mutually Exclusive Roles of SHARPIN in Integrin Inactivation and NF-κB Signaling." in: PLoS ONE, Vol. 10, Issue 11, pp. e0143423, (2015) (PubMed).

Target Details

Target: ITGAL (Integrin alpha-L (ITGAL))

Alternative Name: Cd11a,itgal

Background: ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 126.1 kDa

NCBI Accession: NP_002200

Pathways: Activated T Cell Proliferation, Integrin Complex