PDGFRB Protein

Catalog No. ABIN2713423

This Recombinant PDGFRB protein is expressed in Escherichia coli (E. coli).

Quick Overview for PDGFRB Protein (ABIN2713423)

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Purity: > 98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human CD140b / PDGFRB protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 10-20 ng/ml, corresponding to a specific activity of 1-0.5 x 10^5 units/mg, determined by the dose dependent stimulation of 3T3 cell proliferation. The bioactivity is equivalent to competitor reported values.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 29 % acetonitrile, 0.1 % TFA (trifluoroacetic acid)

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Alternative Name: Cd140b,pdgfrb

Background: This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

Molecular Weight: 12.3 kDa

NCBI Accession: NP_002600

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling