DDR2 Protein (DYKDDDDK Tag)

Catalog No. ABIN2713459

This Recombinant DDR2 protein is expressed in Insect cells (Sf9).

Quick Overview for DDR2 Protein (DYKDDDDK Tag) (ABIN2713459)

Target: DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DDR2 protein is labelled with DYKDDDDK Tag.

Characteristics:

• Recombinant human CD167b / DDR2 (C-term DDK tag, transcript variant 1) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))

Alternative Name: Cd167b,ddr2

Background: Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein.

Molecular Weight: 44.7 kDa

NCBI Accession: NP_001014796

Pathways: RTK Signaling