DDR2 Protein (GST tag,His tag)
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- Target See all DDR2 Proteins
- DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This DDR2 protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Human DDR2 Kinase/CD167b Protein (aa 422-855, His & GST Tag)(Active)
- Sequence
- Arg422-Glu855
- Characteristics
- A DNA sequence encoding the human DDR2 (Q16832) (Arg422-Glu855) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 8 nmol/min/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.
- Top Product
- Discover our top product DDR2 Protein
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- Comment
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77 kDa
- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))
- Alternative Name
- CD167b (DDR2 Products)
- Synonyms
- MIG20a Protein, NTRKR3 Protein, TKT Protein, TYRO10 Protein, AW495251 Protein, Ntrkr3 Protein, tyro10 Protein, DDR2 Protein, Tyro10 Protein, ddr2 Protein, si:ch211-193c2.1 Protein, discoidin domain receptor tyrosine kinase 2 Protein, discoidin domain receptor family, member 2 Protein, discoidin domain receptor family, member 2, like Protein, DDR2 Protein, Ddr2 Protein, ddr2l Protein
- Background
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Background: Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: CD167;MIG20a;NTRKR3;TKT;TYRO10
- Molecular Weight
- 77.1 kDa
- UniProt
- Q16832
- Pathways
- RTK Signaling
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