CD73 Protein (Transcript Variant 1) (His tag)
Quick Overview for CD73 Protein (Transcript Variant 1) (His tag) (ABIN2713773)
Target
See all CD73 (NT5E) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This CD73 protein is labelled with His tag.
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Characteristics
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- Recombinant human CD73 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-554) (Active) protein (His tag)
NT5E Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-547) (Active) protein (hFc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-549) (Active) protein (His tag)NT5E Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (Fc Tag)NT5E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (His tag)NT5E Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) protein (Fc Tag)NT5E Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 27-549) (Active) protein (His tag,AVI tag,Biotin)NT5E Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 28-264) protein (GST tag)NT5E Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
5'-Nucleotidase, Ecto (CD73) (NT5E) protein (His tag)NT5E Origin: Rat Host: Human Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
5'-Nucleotidase, Ecto (CD73) (NT5E) (Active) protein (His tag)NT5E Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 2 mM CaCl2, 10 % Glycerol, pH 8.0
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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Alternative Name
- CD73
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Background
- The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
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Molecular Weight
- 58.76kD
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NCBI Accession
- NP_002517
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Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
Target
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