Quick Overview for CENPH Protein (Myc-DYKDDDDK Tag) (ABIN2713907)
Target
See all CENPH ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This CENPH protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human CENP-H protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Centromere Protein H (CENPH) (AA 1-247) protein (GST tag)
CENPH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Centromere Protein H (CENPH) (AA 136-247) protein (His tag)CENPH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CENPH (Centromere Protein H (CENPH))
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Alternative Name
- Cenp-H
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Background
- Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
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Molecular Weight
- 28.3 kDa
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NCBI Accession
- NP_075060
Target
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