beta Actin Protein (Myc-DYKDDDDK Tag)
Quick Overview for beta Actin Protein (Myc-DYKDDDDK Tag) (ABIN2714357)
Target
See all beta Actin (ACTB) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This beta Actin protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Actin beta / ACTB protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Actin, beta (ACTB) (AA 1-375) protein (His tag)
custom-made ACTB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Actin, beta (ACTB) (AA 1-375) protein (His tag)custom-made ACTB Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Actin, beta (ACTB) (AA 1-375) protein (GST tag)ACTB Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Actin, beta (ACTB) (AA 14-167) protein (His tag)ACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Actin, beta (ACTB) (AA 102-375) protein (His tag)ACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- beta Actin (ACTB) (Actin, beta (ACTB))
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Alternative Name
- Actin Beta,actb
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Background
- This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
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Molecular Weight
- 41.6 kDa
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NCBI Accession
- NP_001092
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Pathways
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
Target
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