AIRE Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714490

This Recombinant AIRE protein is produced in HEK-293 Cells.

Quick Overview for AIRE Protein (Myc-DYKDDDDK Tag) (ABIN2714490)

Target: AIRE (Autoimmune Regulator (AIRE))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This AIRE protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human AIRE / APECED (transcript variant AIRE-1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: Aire,apeced

Background: This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED).

Molecular Weight: 57.5 kDa

NCBI Accession: NP_000374

Pathways: Chromatin Binding