alpha Actinin 4 Protein (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for alpha Actinin 4 Protein (Myc-DYKDDDDK Tag) (ABIN2714614)
Target
See all alpha Actinin 4 (ACTN4) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This alpha Actinin 4 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Alpha-actinin-4 / ACTN4 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Actinin, alpha 4 (ACTN4) (AA 1-911) protein (His tag)
custom-made ACTN4 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Actinin, alpha 4 (ACTN4) (AA 1-912) protein (His tag)custom-made ACTN4 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Actinin, alpha 4 (ACTN4) (AA 1-911) protein (GST tag)ACTN4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Actinin, alpha 4 (ACTN4) (AA 592-701) protein (GST tag)ACTN4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Actinin, alpha 4 (ACTN4) (AA 397-648) protein (His-IF2DI Tag)ACTN4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Alpha-Actinin-4 is a Possible Target Protein for Aristolochic Acid I in Human Kidney Cells In Vitro." in: The American journal of Chinese medicine, Vol. 44, Issue 2, pp. 291-304, (2016) (PubMed).
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: "Alpha-Actinin-4 is a Possible Target Protein for Aristolochic Acid I in Human Kidney Cells In Vitro." in: The American journal of Chinese medicine, Vol. 44, Issue 2, pp. 291-304, (2016) (PubMed).
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- alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))
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Alternative Name
- alpha-Actinin-4,actn4
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Background
- Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
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Molecular Weight
- 104.7 kDa
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NCBI Accession
- NP_004915
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Pathways
- Proton Transport
Target
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