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CHIA Protein (His tag)

This Recombinant CHIA protein is produced in Escherichia coli (E. coli).
Catalog No. ABIN2714649

Quick Overview for CHIA Protein (His tag) (ABIN2714649)

Target

See all CHIA Proteins
CHIA (Chitinase, Acidic (CHIA))

Protein Type

Recombinant

Origin

  • 2
  • 1
  • 1
  • 1
Human

Source

  • 2
  • 2
  • 1
Escherichia coli (E. coli)

Application

Antibody Production (AbP), Standard (STD)

Purity

> 80 % as determined by SDS-PAGE and Coomassie blue staining
  • Purification tag / Conjugate

    This CHIA protein is labelled with His tag.

    Characteristics

    • Recombinant human AMCase (N-term HIS tag, transcript variant 2) protein expressed in E.coli.
    • Produced with end-sequenced ORF clone
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  • Application Notes

    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays

    Comment

    The tag is located at the N-terminal.

    Restrictions

    For Research Use only
  • Concentration

    50 μg/mL

    Buffer

    25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

    Storage

    -80 °C

    Storage Comment

    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target

    CHIA (Chitinase, Acidic (CHIA))

    Alternative Name

    Amcase

    Background

    The protein encoded by this gene degrades chitin, which is found in the cell wall of most fungi as well as in arthropods and some nematodes. The encoded protein can also stimulate interleukin 13 expression, and variations in this gene can lead to asthma susceptibility. Several transcript variants encoding a few different isoforms have been found for this gene.

    Molecular Weight

    40 kDa

    NCBI Accession

    NP_068569
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