CHIA Protein (AA 22-473) (His tag)
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- Target See all CHIA Proteins
- CHIA (Chitinase, Acidic (CHIA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-473
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This CHIA protein is labelled with His tag.
- Purpose
- Mouse AMCase/CHIA Protein
- Sequence
- Tyr22-Pro473
- Characteristics
- Recombinant Mouse AMCase/CHIA Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr22-Pro473.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Chitinase, Acidic (CHIA) (AA 22-476) protein (His tag)
CHIA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chitinase, Acidic (CHIA) (AA 1-368) protein (GST tag)CHIA Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Chitinase, Acidic (CHIA) (AA 22-476) protein (His tag)Crystallography grade CHIA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Chitinase, Acidic (CHIA) (AA 22-473) protein (His tag)Crystallography grade CHIA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Chitinase, Acidic (CHIA) protein (His tag)CHIA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- CHIA (Chitinase, Acidic (CHIA))
- Alternative Name
- AMCase (CHIA Products)
- Synonyms
- chia-a Protein, AMCASE Protein, CHIT2 Protein, TSA1902 Protein, 2200003E03Rik Protein, AMCase Protein, YNL Protein, CHIA Protein, chioIIa Protein, fa55g10 Protein, wu:fa55g10 Protein, zgc:63792 Protein, acidic mammalian chitinase Protein, chitinase, acidic Protein, chitinase, acidic S homeolog Protein, chitinase-M31, acidic Protein, chitinase, acidic 1 Protein, chitinase, acidic.4 Protein, LOC703284 Protein, CHIA Protein, LOC100600050 Protein, chia.S Protein, CHIA-M31 Protein, Chia1 Protein, Chia Protein, chia.4 Protein
- Background
- Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic cells and extremely high levels are seen in lysosomal storage diseases. The expression of AMCase and CHIT-1 were differently modulated in HMMs at different stage of maturation.
- Molecular Weight
- 51 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
- UniProt
- Q91XA9
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