A1CF Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for A1CF Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2714808)
Target
See all A1CF ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This A1CF protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human APOBEC1 complementation factor (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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APOBEC1 Complementation Factor (A1CF) (AA 1-595) protein (His tag)
custom-made A1CF Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
APOBEC1 Complementation Factor (A1CF) (AA 1-594) protein (His tag)custom-made A1CF Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
APOBEC1 Complementation Factor (A1CF) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)A1CF Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- A1CF (APOBEC1 Complementation Factor (A1CF))
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Alternative Name
- Apobec1 Complementation Factor
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Background
- Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Several transcript variants encoding a few different isoforms have been found for this gene.
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Molecular Weight
- 65 kDa
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NCBI Accession
- NP_620310
Target
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