ATP6V1B2 Protein (Myc-DYKDDDDK Tag)
Quick Overview for ATP6V1B2 Protein (Myc-DYKDDDDK Tag) (ABIN2715056)
Target
See all ATP6V1B2 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This ATP6V1B2 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human ATP6V1B2 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2) (AA 1-511) protein (His tag)
custom-made ATP6V1B2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2) (AA 1-511) protein (GST tag)ATP6V1B2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))
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Alternative Name
- Atp6v1b2
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Background
- This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.
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Molecular Weight
- 56.3 kDa
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NCBI Accession
- NP_001684
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Pathways
- Transition Metal Ion Homeostasis, Proton Transport
Target
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