ATP6V1G2 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715062

Recombinant ATP6V1G2 protein expressed in HEK-293 Cells.

Quick Overview for ATP6V1G2 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2715062)

Target: ATP6V1G2 (ATPase, H+ Transporting, Lysosomal 13kDa, V1 Subunit G2 (ATP6V1G2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This ATP6V1G2 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human ATP6V1G2 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ATP6V1G2 (ATPase, H+ Transporting, Lysosomal 13kDa, V1 Subunit G2 (ATP6V1G2))

Alternative Name: Atp6v1g2

Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of three V1 domain G subunit proteins. This gene had previous gene symbols of ATP6G and ATP6G2. Alternatively spliced transcript variants encoding different isoforms have been described. Read-through transcription also exists between this gene and the downstream DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B (DDX39B) gene.

Molecular Weight: 8.8 kDa

NCBI Accession: NP_612139

Pathways: Transition Metal Ion Homeostasis, Proton Transport