Axin Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715088

This Recombinant Axin protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for Axin Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2715088)

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This Axin protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Axin-1 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Cha, Cho, Lee, Lee, Jeong, Moon, Yun, Yang, Choi, Yoon, Kim, Kim, Kaduwal, Lee, Min, Kim, Han, Choi: "Small-molecule binding of the axin RGS domain promotes β-catenin and Ras degradation." in: Nature chemical biology, Vol. 12, Issue 8, pp. 593-600, (2016) (PubMed).

Target Details

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Alternative Name: Axin-1

Background: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants.

Molecular Weight: 91.5 kDa

NCBI Accession: NP_851393

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling