BCDIN3D Protein (Myc-DYKDDDDK Tag)
Quick Overview for BCDIN3D Protein (Myc-DYKDDDDK Tag) (ABIN2715187)
Target
See all BCDIN3D ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This BCDIN3D protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human BCDIN3D protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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BCDIN3 Domain Containing (BCDIN3D) (AA 1-285) protein (His tag)
custom-made BCDIN3D Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCDIN3 Domain Containing (BCDIN3D) (AA 1-292) protein (His tag)BCDIN3D Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
BCDIN3 Domain Containing (BCDIN3D) (AA 1-292) protein (His-IF2DI Tag)BCDIN3D Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- BCDIN3D (BCDIN3 Domain Containing (BCDIN3D))
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Alternative Name
- Bcdin3d
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Background
- This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family, and serves as a 5&apos-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs.
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Molecular Weight
- 33 kDa
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NCBI Accession
- NP_859059
Target
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