Beclin 1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715218

Recombinant Beclin 1 protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for Beclin 1 Protein (Myc-DYKDDDDK Tag) (ABIN2715218)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Beclin 1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Beclin-1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Zhu, Messer, Wang, Lin, Cham, Chang, Billiar, Lotze, Boone, Chang: "Cytosolic HMGB1 controls the cellular autophagy/apoptosis checkpoint during inflammation." in: The Journal of clinical investigation, Vol. 125, Issue 3, pp. 1098-110, (2015) (PubMed).

Target Details

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin-1

Background: This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Molecular Weight: 51.7 kDa

NCBI Accession: NP_003757

Pathways: Autophagy