HLCS Protein (Myc-DYKDDDDK Tag)
Quick Overview for HLCS Protein (Myc-DYKDDDDK Tag) (ABIN2715276)
Target
See all HLCS ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This HLCS protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Biotin-protein ligase / HLCS protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Biotin-Protein Ligase (HLCS) (AA 1-726) protein (His tag)
custom-made HLCS Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Biotin-Protein Ligase (HLCS) (AA 1-722) protein (His tag)custom-made HLCS Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Biotin-Protein Ligase (HLCS) (AA 1-726) protein (GST tag)HLCS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- HLCS (Biotin-Protein Ligase (HLCS))
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Alternative Name
- Biotin-Protein Ligase,hlcs
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Background
- This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011].
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Molecular Weight
- 80.6 kDa
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NCBI Accession
- NP_000402
Target
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