HLCS Protein (AA 1-726) (GST tag)
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- Target See all HLCS Proteins
- HLCS (Biotin-Protein Ligase (HLCS))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-726
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This HLCS protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- HLCS (Human) Recombinant Protein (P01)
- Sequence
- MEDRLHMDNG LVPQKIVSVH LQDSTLKEVK DQVSNKQAQI LEPKPEPSLE IKPEQDGMEH VGRDDPKALG EEPKQRRGSA SGSEPAGDSD RGGGPVEHYH LHLSSCHECL ELENSTIESV KFASAENIPD LPYDYSSSLE SVADETSPER EGRRVNLTGK APNILLYVGS DSQEALGRFH EVRSVLADCV DIDSYILYHL LEDSALRDPW TDNCLLLVIA TRESIPEDLY QKFMAYLSQG GKVLGLSSSF TFGGFQVTSK GALHKTVQNL VFSKADQSEV KLSVLSSGCR YQEGPVRLSP GRLQGHLENE DKDRMIVHVP FGTRGGEAVL CQVHLELPPS SNIVQTPEDF NLLKSSNFRR YEVLREILTT LGLSCDMKQV PALTPLYLLS AAEEIRDPLM QWLGKHVDSE GEIKSGQLSL RFVSSYVSEV EITPSCIPVV TNMEAFSSEH FNLEIYRQNL QTKQLGKVIL FAEVTPTTMR LLDGLMFQTP QEMGLIVIAA RQTEGKGRGG NVWLSPVGCA LSTLLISIPL RSQLGQRIPF VQHLMSVAVV EAVRSIPEYQ DINLRVKWPN DIYYSDLMKI GGVLVNSTLM GETFYILIGC GFNVTNSNPT ICINDLITEY NKQHKAELKP LRADYLIARV VTVLEKLIKE FQDKGPNSVL PLYYRYWVHS GQQVHLGSAE GPKVSIVGLD DSGFLQVHQE GGEVVTVHPD GNSFDMLRNL ILPKRR
- Characteristics
- Human HLCS full-length ORF ( NP_000402.3, 1 a.a. - 726 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Biotin-Protein Ligase (HLCS) (AA 627-724) protein (GST tag)
HLCS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Biotin-Protein Ligase (HLCS) (AA 1-726) protein (Strep Tag)Crystallography grade HLCS Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Biotin-Protein Ligase (HLCS) (AA 1-722) protein (Strep Tag)Crystallography grade HLCS Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Biotin-Protein Ligase (HLCS) protein (Myc-DYKDDDDK Tag)HLCS Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- HLCS (Biotin-Protein Ligase (HLCS))
- Alternative Name
- HLCS (HLCS Products)
- Synonyms
- 410I21.SP6 Protein, D16Jhu34 Protein, HCS Protein, RGD1565360 Protein, holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) Protein, holocarboxylase synthetase Protein, Hlcs Protein, HLCS Protein
- Background
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Full Gene Name: holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase (ATP-hydrolysing)) ligase)
Synonyms: HCS - Gene ID
- 3141
- NCBI Accession
- NM_000411
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