BMP4 Protein (Transcript Variant 1)
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- Target See all BMP4 Proteins
- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human BMP4 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 5-10 ng/ml.
- Top Product
- Discover our top product BMP4 Protein
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- BMP4 (Bone Morphogenetic Protein 4 (BMP4))
- Alternative Name
- Bmp4 (BMP4 Products)
- Synonyms
- BMP2B Protein, BMP2B1 Protein, MCOPS6 Protein, OFC11 Protein, ZYME Protein, Bmp-4 Protein, Bmp2b Protein, Bmp2b-1 Protein, Bmp2b1 Protein, BOMPR4A Protein, bmp-4 Protein, zbmp-4 Protein, zgc:100779 Protein, BMP-4 Protein, XBMP-4 Protein, bmp2b Protein, bmp2b1 Protein, bmp4 Protein, ofc11 Protein, xbmp4 Protein, zyme Protein, BMP4 Protein, bone morphogenetic protein 4 Protein, bone morphogenetic protein 4 L homeolog Protein, bone morphogenetic protein 4 S homeolog Protein, BMP4 Protein, Bmp4 Protein, bmp4 Protein, bmp4.L Protein, bmp4.S Protein
- Background
- This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers.
- Molecular Weight
- 12 kDa
- NCBI Accession
- NP_001193
- Pathways
- Steroid Hormone Mediated Signaling Pathway, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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