BRAP Protein (His tag)

Catalog No. ABIN2715326

Recombinant BRAP protein expressed in Escherichia coli (E. coli).

Quick Overview for BRAP Protein (His tag) (ABIN2715326)

Target: BRAP (BRCA1 Associated Protein (BRAP))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This BRAP protein is labelled with His tag.

Characteristics:

• Recombinant human BRAP / RNF52 (N-term HIS tag) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: BRAP (BRCA1 Associated Protein (BRAP))

Alternative Name: Brap,rnf52

Background: The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm.

Molecular Weight: 67.1 kDa

NCBI Accession: NP_006759