C12orf65 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715398

Recombinant C12orf65 protein expressed in HEK-293 Cells.

Quick Overview for C12orf65 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2715398)

Target: C12orf65 (Chromosome 12 Open Reading Frame 65 (C12orf65))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This C12orf65 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human C12orf65 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: C12orf65 (Chromosome 12 Open Reading Frame 65 (C12orf65))

Alternative Name: c12orf65

Background: This nuclear gene encodes a mitochondrial matrix protein that appears to contribute to peptide chain termination in the mitochondrial translation machinery. Two different 1 bp deletions (resulting in the same premature stop codon)result in decreased mitochondrial translation, decreased levels of oxidative phosphorylation complexes and encepthalomyopathy. Alternative splicing results in multiple transcript variants.

Molecular Weight: 18.6 kDa

NCBI Accession: NP_689482