DGKD Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715543

This Recombinant DGKD protein is produced in HEK-293 Cells.

Quick Overview for DGKD Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2715543)

Target: DGKD (Diacylglycerol Kinase, delta 130kDa (DGKD))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This DGKD protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DAG kinase delta (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DGKD (Diacylglycerol Kinase, delta 130kDa (DGKD))

Alternative Name: Dag Kinase delta

Background: This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms.

Molecular Weight: 134.3 kDa

NCBI Accession: NP_690618

Pathways: EGFR Signaling Pathway