HSD11B1 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- Target See all HSD11B1 Proteins
- HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HSD11B1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human 11-beta HSD1 / HSD11B1 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product HSD11B1 Protein
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Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1) (AA 196-292) protein (His-IF2DI Tag)
HSD11B1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1) (AA 1-292) protein (GST tag)HSD11B1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1) (AA 25-292) protein (His-SUMO Tag)HSD11B1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)HSD11B1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))
- Alternative Name
- 11-beta Hsd1,hsd11b1 (HSD11B1 Products)
- Background
- The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011]
- Molecular Weight
- 32.2 kDa
- NCBI Accession
- NP_861420
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Carbohydrate Metabolic Process
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