APITD1 Protein (Transcript Variant B) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2716304

Recombinant APITD1 protein expressed in HEK-293 Cells.

Quick Overview for APITD1 Protein (Transcript Variant B) (Myc-DYKDDDDK Tag) (ABIN2716304)

Target: APITD1 (Apoptosis-Inducing, TAF9-Like Domain 1 (APITD1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant B

Purification tag / Conjugate: This APITD1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Apoptosis-inducing, TAF9-like domain 1 (APITD1), transcript variant B (transcript variant B) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: APITD1 (Apoptosis-Inducing, TAF9-Like Domain 1 (APITD1))

Background: This gene was identified in the neuroblastoma tumor suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumors, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway. The protein is a component of multiple complexes, including the Fanconi anemia (FA) core complex, the APITD1/CENPS complex, and the CENPA-CAD (nucleosome distal) complex. Known functions include an involvement with chromatin associations of the FA core complex, and a role in the stable assembly of the outer kinetochore. Alternative splicing of this gene results in multiple transcript variants. Naturally occurring read-through transcripts also exist between this gene and the downstream cortistatin (CORT) gene, as represented in GeneID:100526739. An APITD1-related pseudogene has been identified on chromosome 7.

Molecular Weight: 11.1 kDa

NCBI Accession: NP_954992