DBT Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719240

This Recombinant DBT protein is expressed in HEK-293 Cells.

Quick Overview for DBT Protein (Myc-DYKDDDDK Tag) (ABIN2719240)

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DBT protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DBT protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Alternative Name: Dbt

Target Type: Viral Protein

Background: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 46.4 kDa

NCBI Accession: NP_001909