DENND1C Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719381

This Recombinant DENND1C protein is expressed in HEK-293 Cells.

Quick Overview for DENND1C Protein (Myc-DYKDDDDK Tag) (ABIN2719381)

Target: DENND1C (DENN/MADD Domain Containing 1C (DENND1C))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DENND1C protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DENND1C protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DENND1C (DENN/MADD Domain Containing 1C (DENND1C))

Background: Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1C, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2 see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010].

Molecular Weight: 86.9 kDa

NCBI Accession: NP_079174