Dihydrofolate Reductase Protein (DHFR) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719443

This Recombinant Dihydrofolate Reductase protein is produced in HEK-293 Cells.

Quick Overview for Dihydrofolate Reductase Protein (DHFR) (Myc-DYKDDDDK Tag) (ABIN2719443)

Target: Dihydrofolate Reductase (DHFR)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This Dihydrofolate Reductase protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DHFR / DHFRP1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: Dhfr,dhfrp1

Background: Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 21.3 kDa

NCBI Accession: NP_000782

Pathways: Mitotic G1-G1/S Phases