DHODH Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719446

This Recombinant DHODH protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for DHODH Protein (Myc-DYKDDDDK Tag) (ABIN2719446)

Target: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DHODH protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DHOdehase protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Sykes, Kfoury, Mercier, Wawer, Law, Haynes, Lewis, Schajnovitz, Jain, Lee, Meyer, Pierce, Tolliday, Waller, Ferrara, Eheim, Stoeckigt, Maxcy, Cobert, Bachand, Szekely, Mukherjee, Sklar, Kotz, Clish et al.: "Inhibition of Dihydroorotate Dehydrogenase Overcomes Differentiation Blockade in Acute Myeloid Leukemia. ..." in: Cell, Vol. 167, Issue 1, pp. 171-186.e15, (2016) (PubMed).

Target Details

Target: DHODH (Dihydroorotate Dehydrogenase (DHODH))

Alternative Name: Dhodehase

Background: Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 41.6 kDa

NCBI Accession: NP_001352

Pathways: Ribonucleoside Biosynthetic Process, Protein targeting to Nucleus