DIRAS3 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719498

This Recombinant DIRAS3 protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for DIRAS3 Protein (Myc-DYKDDDDK Tag) (ABIN2719498)

Target: DIRAS3 (DIRAS Family, GTP-Binding RAS-Like 3 (DIRAS3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DIRAS3 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DIRAS3 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Zhang, Lu, Mao, Ahmed, Yang, Zhou, Jennings, Rodriguez-Aguayo, Lopez-Berestein, Miranda, Qiao, Baladandayuthapani, Li, Sood, Liu, Le, Bast: "CDK5 Regulates Paclitaxel Sensitivity in Ovarian Cancer Cells by Modulating AKT Activation, p21Cip1- and p27Kip1-Mediated G1 Cell Cycle Arrest and Apoptosis." in: PLoS ONE, Vol. 10, Issue 7, pp. e0131833, (2015) (PubMed).

Target Details

Target: DIRAS3 (DIRAS Family, GTP-Binding RAS-Like 3 (DIRAS3))

Alternative Name: Diras3

Background: This gene encodes a member of the ras superfamily. This gene is imprinted gene with monoallelic expression of the paternal allele which is associated with growth suppression. The encoded protein acts as a tumor suppressor whose function is abrogated in many ovarian and breast cancers. This protein may also play a role autophagy in certain cancer cells by regulating the autophagosome initiation complex.

Molecular Weight: 25.7 kDa

NCBI Accession: NP_004666