LIG3 Protein (Myc-DYKDDDDK Tag)
This Recombinant LIG3 protein is expressed in HEK-293 Cells.
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Catalog No. ABIN2719546
$1,112.40
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20 μg
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United States
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Quick Overview for LIG3 Protein (Myc-DYKDDDDK Tag) (ABIN2719546)
Target
See all LIG3 Proteins
LIG3
(Ligase III, DNA, ATP-Dependent (LIG3))
Protein Type
Recombinant
Origin
Human
Source
HEK-293 Cells
Application
Antibody Production (AbP), Standard (STD)
Purity
> 80 % as determined by SDS-PAGE and Coomassie blue staining
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Purification tag / Conjugate
- This LIG3 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human DNA ligase 3 (transcript variant alpha) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Ligase III, DNA, ATP-Dependent (LIG3) (AA 1-299) protein (GST tag)
LIG3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))
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Alternative Name
- Dna Ligase 3
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Background
- This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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Molecular Weight
- 108.6 kDa
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NCBI Accession
- NP_039269
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Pathways
- DNA Damage Repair
Target
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