DNMT3B Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719618

This Recombinant DNMT3B protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for DNMT3B Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2719618)

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This DNMT3B protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DNMT3B (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 10 mM NaCl, 20 mM Phosphate buffer, pH 7.6

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Cree, Fredericks, Miller, Pearce, Filichev, Fee, Kennedy: "DNA G-quadruplexes show strong interaction with DNA methyltransferases in vitro." in: FEBS letters, Vol. 590, Issue 17, pp. 2870-83, (2016) (PubMed).

Target Details

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Alternative Name: Dnmt3b

Molecular Weight: 95.6 kDa

NCBI Accession: NP_008823