DRAP1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719758

This Recombinant DRAP1 protein is produced in HEK-293 Cells.

Quick Overview for DRAP1 Protein (Myc-DYKDDDDK Tag) (ABIN2719758)

Target: DRAP1 (DR1-Associated Protein 1 (Negative Cofactor 2 Alpha) (DRAP1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DRAP1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DRAP1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DRAP1 (DR1-Associated Protein 1 (Negative Cofactor 2 Alpha) (DRAP1))

Alternative Name: Drap1

Background: Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex.

Molecular Weight: 22.2 kDa

NCBI Accession: NP_006433