Deoxyuridine Triphosphatase (DUT) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)
Quick Overview for Deoxyuridine Triphosphatase (DUT) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag) (ABIN2719842)
Target
See all Deoxyuridine Triphosphatase (DUT) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 3
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Purification tag / Conjugate
- Myc-DYKDDDDK Tag
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Characteristics
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- Recombinant human DUT (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Deoxyuridine Triphosphatase (DUT) (AA 1-252) protein (GST tag)
DUT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Deoxyuridine Triphosphatase (DUT) (AA 1-164) protein (His-IF2DI Tag)DUT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Deoxyuridine Triphosphatase (DUT) (AA 70-252) protein (His-IF2DI Tag)DUT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Deoxyuridine Triphosphatase (DUT) (AA 70-252) protein (His tag)DUT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Deoxyuridine Triphosphatase (DUT) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DUT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Deoxyuridine Triphosphatase (DUT) (AA 1-278), (full length) protein (His-SUMO Tag)DUT Origin: Epstein-Barr Virus (EBV) Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Deoxyuridine Triphosphatase (DUT)
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Alternative Name
- Dut
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Target Type
- Viral Protein
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Background
- This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
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Molecular Weight
- 15.2 kDa
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NCBI Accession
- NP_001020420
Target
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