ECH1 Protein (Myc-DYKDDDDK Tag)
Quick Overview for ECH1 Protein (Myc-DYKDDDDK Tag) (ABIN2719918)
Target
See all ECH1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This ECH1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human ECH1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))
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Alternative Name
- Ech1
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Background
- This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
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Molecular Weight
- 35.6 kDa
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NCBI Accession
- NP_001389
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Pathways
- Monocarboxylic Acid Catabolic Process
Target
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