EIF2S2 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720060

Recombinant EIF2S2 protein expressed in HEK-293 Cells.

Quick Overview for EIF2S2 Protein (Myc-DYKDDDDK Tag) (ABIN2720060)

Target: EIF2S2 (Eukaryotic Translation Initiation Factor 2, Subunit 2 Beta, 38kDa (EIF2S2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This EIF2S2 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human EIF2B / EIF2S2 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: EIF2S2 (Eukaryotic Translation Initiation Factor 2, Subunit 2 Beta, 38kDa (EIF2S2))

Alternative Name: Eif2b,eif2s2

Background: EIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 38.2 kDa

NCBI Accession: NP_003899